Gallo et al. J Cell Sci. 2020


Role of the Sec22b-E-Syt complex in neurite growth and ramification. Gallo A, Danglot L, Giordano F, Hewlett B, Binz T, Vannier C, Galli T. J Cell Sci. 2020 Sep 15;133(18):jcs247148. doi: 10.1242/jcs.247148. PMID: 32843578. Download from HAL-INSERM


Axons and dendrites are long and often ramified neurites that need particularly intense plasma membrane (PM) expansion during the development of the nervous system. Neurite growth depends on non-fusogenic Sec22b-Stx1 SNARE complexes at endoplasmic reticulum (ER)-PM contacts. Here, we show that Sec22b interacts with members of the extended synaptotagmin (E-Syt) family of ER lipid transfer proteins (LTPs), and this interaction depends on the longin domain of Sec22b. Overexpression of E-Syts stabilizes Sec22b-Stx1 association, whereas silencing of E-Syts has the opposite effect. Overexpression of wild-type E-Syt2, but not mutants unable to transfer lipids or attach to the ER, increase the formation of axonal filopodia and ramification of neurites in developing neurons. This effect is inhibited by a clostridial neurotoxin cleaving Stx1, and expression of the Sec22b longin domain and a Sec22b mutant with an extended linker between the SNARE and transmembrane domains. We conclude that Sec22b-Stx1 ER-PM contact sites contribute to PM expansion by interacting with LTPs, such as E-Syts.This article has an associated First Person interview with the first author of the paper.

Keywords: Axonal growth; Filopodia; Lipid transfer protein; Membrane contact site; Membrane trafficking; SNARE.