Team leader : Zsolt Lenkei
The team’s previous projects were mainly focused to decipher the closely intertwined relationship between cannabinoid receptor activation and sub-neuronal targeting, by using quantitative in vitro imaging approaches of neurons. In the last years, we became increasingly interested in the understanding of cannabinoid-mediated regulation of neuronal structure. Established techniques range from molecular constructions through imaging-based measurements of GPCR activation in cultured hippocampal neurons to the use of animal models of cerebral plasticity.
Current projects of the team are targeted to develop and use new tools to better understand, at multiple spatio-temporal scales, the role of the highly dynamic actomyosin cytoskeleton in neuronal function and neuropsychiatric pathogenesis. One of this new tools, Ultrafast functional Ultrasound (fUS) imaging, also revealed itself as highly adapted to access nervous system function through imaging the neurovascular coupling, so an important part of our current efforts is focused to further develop this powerful tool either in collaborative projects or in-house, the latter focused on imaging the functional consequences of actomyosin remodeling on brain structure and connectivity.
1 / Kim Y, Vadodaria KC, Lenkei Z, Kato T, Gage FH, Marchetto MC and Santos R (2019) Mitochondria, metabolism and redox mechanisms in psychiatric disorders. Antioxid Redox Signal. doi: 10.1089/ars.2018.7606.
2 / Chatelin S, Humbert-Claude M, Garteiser P, Ricobaraza A, Vilgrain V, Van Beers BE, Sinkus R, Lenkei Z. Cannabinoid receptor activation in the juvenile rat brain results in rapid biomechanical alterations: Neurovascular mechanism as a putative confounding factor. J Cereb Blood Flow Metab. 2016 May;36(5):954-64. doi: 10.1177/0271678X15606923.
3 / Errico C, Pierre J, Pezet S, Desailly Y, Lenkei Z, Couture O, Tanter M. Ultrafast ultrasound localization microscopy for deep super-resolution vascular imaging. Nature. 2015 Nov 26;527(7579):499-502. doi: 10.1038/nature16066.
4 / AB Roland, A Ricobaraza, D Carrel, BM Jordan, F Rico, A Simon, M Humbert-Claude, J Ferrier, MH McFadden, S Scheuring, Z Lenkei Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth. eLife 2014;3:e03159 doi: 10.7554/
5 / Osmanski BF, Pezet S, Ricobaraza A, Lenkei Z, Tanter M. Functional ultrasound imaging of intrinsic connectivity in the living rat brain with high spatiotemporal resolution. Nat Commun. 2014 Oct 3;5:5023. doi: 10.1038/ncomms6023.